Comprehensive Respiratory Sample Collection

Deep cough swabs were collected after obligate coughs using sterile cotton swabs and placed in DNA LoBind Tubes (Eppendorf; no. 30120094) (40 (link)). The swabs were trimmed with sterile scissors and immediately stored at −80°C until further processing. Sputum was collected according to the CFFT Therapeutics Development Network standard operating procedure 530.00 (70 ) for sputum induction after inhalation of 3% NaCl in sterile tubes (Sarstedt; no. 62.547.004) and immediately stored at −80°C until further processing.

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Pallenberg S.T., Pust M.M., Rosenboom I., Hansen G., Wiehlmann L., Dittrich A.M, & Tümmler B. (2022). Impact of Elexacaftor/Tezacaftor/Ivacaftor Therapy on the Cystic Fibrosis Airway Microbial Metagenome. Microbiology Spectrum, 10(5), e01454-22.

Publication 2022

DNA LoBind Tubes sterile tubes

Cotton Coughs Inhalation Nacl Sputum Sterile

Corresponding Organization :

Other organizations : German Center for Lung Research, Medizinische Hochschule Hannover

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Variable analysis

independent variables

Type of sample collection (deep cough swabs or sputum)

dependent variables

Not explicitly mentioned

control variables

Use of sterile cotton swabs and sterile tubes
Immediate storage of samples at -80°C until further processing

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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