Human lung cancer cells (A549 and NCI-H1650) and normal lung cells (NL-20) were incubated with the indicated concentrations of STM for 24 h in 96-well plates and cell viability was determined by MTT assay as described by us previously [16 (link)]. Drug treated cells were then incubated with 10 μL MTT reagent (5 mg/mL) at 37°C for 4 h. Subsequently, the medium was removed and 150 μL DMSO was added to dissolve farmazan crystals. The absorbance was measured at 570 nm (A570) by a microplate reader (Synergy Neo HTS multimode microplate reader, BioTek) and the percentage of cell viability was calculated as follows:
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