Flow cytometric analysis was performed in accordance with previously described methods [15 (link)]. An anti-human CD44 IgG antibody (BioLegend, San Diego, CA, 338804, 1:20), anti-human CD73 IgG antibody (BioLegend, 344004, 1:20), anti-human CD90 IgG antibody (BioLegend, 328108, 1:20), anti-human CD11b IgG antibody (BioLegend, 301404, 1:20), anti-human CD34 IgG antibody (BioLegend, 343504, 1:20), anti-human CD45 IgG antibody (BioLegend, 304006, 1:20), anti-human HLA-A–C IgG antibody (BioLegend, 311404, 1:20), and anti-human HLA-DR IgG antibody (BioLegend, 307604, 1:20) were used. Stained ASCs were analyzed by a BD FACSVerse (Becton, Dickinson and Company, Franklin Lakes, NJ). The data were evaluated by FlowJo software (FlowJo, LLC, Ashland, OR).
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