HPLC Analysis of Glucose and Derivatives

Samples were analyzed by reversed-phase HPLC according to the method described by Hollenbach et al., 2020 [24 (link)]. The HPLC system was equipped with a Kinetex EVO C18 column (2.6 μm, 250 × 4.6 mm) from Phenomenex (Aschaffenburg, Germany) with an accompanying guard column (4 × 3.0 mm ID) of the same phase using an Agilent (Waldbronn, Germany)1260 series liquid chromatograph equipped with a quaternary pump, an autosampler and a column oven. Analytes were detected using an evaporative light scattering detector from BÜCHI Labortechnik (Essen, Germany). The retention times were 2.1 min for glucose and 2.7 min for glucose monodecanoate.

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Hollenbach R., Ochsenreither K, & Syldatk C. (2020). Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent. International Journal of Molecular Sciences, 21(12), 4342.

Publication 2020

Kinetex EVO C18 column 1260 series liquid chromatograph evaporative light scattering detector

Glucose Hplc Light Liquid chromatograph Retention

Corresponding Organization : Karlsruhe Institute of Technology

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Variable analysis

independent variables

Reversed-phase HPLC method according to Hollenbach et al., 2020 [24]

dependent variables

Retention times of glucose (2.1 min)
Retention times of glucose monodecanoate (2.7 min)

control variables

Kinetex EVO C18 column (2.6 μm, 250 × 4.6 mm) from Phenomenex (Aschaffenburg, Germany) with an accompanying guard column (4 × 3.0 mm ID) of the same phase
Agilent (Waldbronn, Germany) 1260 series liquid chromatograph equipped with a quaternary pump, an autosampler and a column oven
Evaporative light scattering detector from BÜCHI Labortechnik (Essen, Germany)

controls

No positive or negative controls were explicitly mentioned.

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