Evaluating Oxidative Stress in ICH

ROS were evaluated after ICH via in situ detection of oxidized Hydroethidine [74 (link)]. Hydroethidine (Thermo Fisher Scientific) was dissolved in DMSO and then diluted in PBS to a final concentration of 1 mg/mL. Three hundred milliliters of Hydroethidine were injected intraperitoneally on day 3 after ICH, 2 hours before the brains were harvested. Fluorescence intensity of predefined areas of the hemorrhagic striatum in the brain sections was determined. An investigator blinded to the experimental groups analyzed the sections using ImageJ software.

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Wang Y., Song Y., Pang Y., Yu Z., Hua W., Gu Y., Qi J, & Wu H. (2020). miR-183-5p alleviates early injury after intracerebral hemorrhage by inhibiting heme oxygenase-1 expression. Aging (Albany NY), 12(13), 12869-12895.

Publication 2020

Hydroethidine

Brain Brains 2 Dmso Fluorescence Hemorrhagic Hydroethidine Striatum

Corresponding Organization :

Other organizations : Harbin Medical University, First Affiliated Hospital of Harbin Medical University

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Variable analysis

independent variables

Time after ICH (day 3)

dependent variables

Fluorescence intensity of predefined areas of the hemorrhagic striatum in the brain sections

control variables

Hydroethidine concentration (1 mg/mL)
Hydroethidine administration (300 mL intraperitoneally, 2 hours before brain harvesting)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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