Phospholipid Profiling in Mouse Organs

Phospholipid standards for the assignment of phospholipid signals in the ³¹P NMR experiments on the mouse organs (the brain, liver, and kidney) and the human lymphoblast samples were the same as in the previous report on the mouse heart and are summarized therein (Kimura et al, 2018 (link)). Detergents: SDS and sodium cholate hydrate used to dissolve lipids in water for ³¹P NMR measurements were from Bioshop Canada and Sigma-Aldrich, respectively. SDS was used regularly in the phospholipid quantification, whereas cholate was used to confirm the absence of a detectable level of lysoplasmalogen (Kimura et al, 2018 (link)). Buffer: MES hydrate and 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (EPPS) used to prepare the buffered (at pH = 6.0 and 8.5, respectively) SDS micellar solution were from Sigma-Aldrich and Sigma. EDTA and cesium hydroxide hydrate or a cesium hydroxide solution used to prepare the Cs-EDTA aqueous solution to homogenize the mouse organs and the human lymphoblast were from Sigma-Aldrich. An antioxidant, butylated hydroxytoluene (BHT) was from Sigma-Aldrich. Solvent: methanol (HPLC grade) and chloroform (HPLC grade) were from Sigma-Aldrich. NMR solvent deuterium oxide (99.9 atom %D) was from Cambridge Isotope Laboratories.