The basal hsp68 promoter of PCR-linearized p130der was replaced with the TC006550 promoter amplified from blaAmp-Tc6550Pro-GFPZeo-Luciferase-HSP-Orange-pIZT (kindly provided by Dr. Yoonseong Park) [13 (link)] with NEBuilder HiFi assembly (NEB). The resulting pTC006550-GAL4Δ-SV40 polyA coding sequence was then amplified and inserted into PCR-linearized pBac[3xP3-EGFP] with NEBuilder HiFi assembly. (DGRC # 1427)
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