Immunohistochemical Analysis of Tumor Xenografts

Tissue sections (4-μm) from formalin-fixed, paraffin-embedded tumor xenografts were immunostained with anti-Ki67 and –active caspase-3 antibodies (1:100 dilution) using the MaxVision kit (Maixin, Fuzhou, China) according to the standard protocol as described [23 (link), 28 (link)]. Color was developed with 0.05% diaminobenzidine and the sections were counterstained with hematoxylin. The sections were mounted with Permount™ Mounting Medium and photographed under an inverted fluorescence microscope.

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Zhang J., Liu S., Ye Q, & Pan J. (2019). Transcriptional inhibition by CDK7/9 inhibitor SNS-032 abrogates oncogene addiction and reduces liver metastasis in uveal melanoma. Molecular Cancer, 18, 140.

Publication 2019

MaxVision kit

Antibodies Caspase 3 Dilution Fluorescence microscope Formalin Hematoxylin Paraffin Tissue Tumor Xenografts

Corresponding Organization :

Other organizations : Sun Yat-sen University

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Variable analysis

independent variables

Tissue sections (4-μm) from formalin-fixed, paraffin-embedded tumor xenografts

dependent variables

Ki67 immunostaining
Active caspase-3 immunostaining

control variables

Tissue section thickness (4-μm)
Formalin-fixation and paraffin-embedding of tumor xenografts
Antibody dilution (1:100)
Use of MaxVision kit for immunostaining
Color development with 0.05% diaminobenzidine
Counterstaining with hematoxylin
Mounting with Permount™ Mounting Medium
Imaging under an inverted fluorescence microscope

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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