Characterization of CRC Cell Lines

The human colorectal tumor cell lines HCT116, DLD1, SW480, SW620, SW837, and HT29 were obtained from ATCC. HCT116 cells line was used as in vitro model due to wildtype Tp53 status compared to other CRC cell lines (Supplementary Table 1). HCT116 and HCT116-derived monoclonal DAPK1 ko clones 7/6, 10/8 and 21/9 were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1% penicillin (100 U/ml) and streptomycin (100 µg/ml) (all from PAN Biotech, Aidenbach, Germany) and cultured in a humidified atmosphere of 5% CO₂ at 37 °C. For ERK1/2 inactivation, cells were treated with 10 and 20 µM FR180204 (Selleckchem, Munich, Germany) for 48 h. All cell lines were genotyped using Multiplex Cell Authentication by Multiplexion (Heidelberg, Germany) as described recently^{15 (link)}. Mycoplasma-free status has been verified for all cell lines.