Immunodetection of Epitope-Tagged Proteins in Toxoplasma

The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (BioLegend, San Diego, CA) or rabbit polyclonal antibody (pAb) anti-HA (ThermoFisher). The Ty1 epitope was detected with mouse mAb BB2 [57 (link)]. The c-Myc epitope was detected with mouse mAb 9E10 [58 (link)] or rabbit pAb anti-Myc (ThermoFisher). The V5 epitope was detected with mouse mAb anti-V5 (ThermoFisher). Toxoplasma-specific antibodies include rabbit pAb anti-SAG1 [59 (link)], mouse mAb anti-IMC1 [60 (link)], mouse pAb anti-ISP3 [61 (link)], and mouse mAb anti-ROP7 [62 (link)]. Production of rat pAb anti-ILP1 and rabbit pAb anti-IMC6 is described below.

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Choi C.P., Moon A.S., Back P.S., Jami‐Alahmadi Y., Vashisht A.A., Wohlschlegel J.A, & Bradley P.J. (2019). A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex. PLoS Biology, 17(10), e3000475.

Publication 2019

HA.11 pAb) anti-HA anti-Myc anti-V5

Anti antibody Antibodies Antibody Antibody production C myc Epitope Monoclonal antibody Mouse Rabbit Toxoplasma

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (BioLegend, San Diego, CA) or rabbit polyclonal antibody (pAb) anti-HA (ThermoFisher)
The Ty1 epitope was detected with mouse mAb BB2
The c-Myc epitope was detected with mouse mAb 9E10 or rabbit pAb anti-Myc (ThermoFisher)
The V5 epitope was detected with mouse mAb anti-V5 (ThermoFisher)
Toxoplasma-specific antibodies include rabbit pAb anti-SAG1, mouse mAb anti-IMC1, mouse pAb anti-ISP3, and mouse mAb anti-ROP7

dependent variables

Detection of the HA, Ty1, c-Myc, and V5 epitopes
Detection of Toxoplasma-specific antibodies

control variables

Production of rat pAb anti-ILP1 and rabbit pAb anti-IMC6 is described below

positive controls

Production of rat pAb anti-ILP1 and rabbit pAb anti-IMC6

Annotations

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