Serum and fecal anti-dsDNA IgG, IgM and IgA were quantified by ELISA with 1:100 dilutions as previously described14 (link). Anti-dsDNA IgG were also visualized on Crithidia luciliae slides (Bio-Rad) with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus). The corrected total cell fluorescence (https://theolb.readthedocs.io/en/latest/# ) from each Crithidia luciliae cell was quantified with ImageJ. IL-6 or IFN-γ were quantitated with BD Biosciences ELISA kits in serum samples diluted 1:50 assayed in duplicate. The absorbance was detected with the Promega GloMax® Explorer microplate reader at 450 nm.
Protocol detail
Quantification of Anti-dsDNA Autoantibodies
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : The University of Texas Health Science Center at San Antonio, University of Florida
Top 5 similar protocols
Variable analysis
independent variables
- Serum and fecal samples
- Anti-dsDNA IgG, IgM and IgA levels quantified by ELISA
- Anti-dsDNA IgG visualization on Crithidia luciliae slides
- Corrected total cell fluorescence from Crithidia luciliae cells
- IL-6 or IFN-γ levels quantified by ELISA
- Serum and fecal samples diluted as specified (1:100 for ELISA, 1:10 for Crithidia luciliae)
- Goat anti-mouse IgG-FITC (1:100) used for Crithidia luciliae slides
- ELISA kits used for IL-6 and IFN-γ quantification
- Promega GloMax® Explorer microplate reader used to detect absorbance at 450 nm
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!