LC/MS was conducted as in [32 (link)]. Briefly, samples were homogenized in 2 ml of acetonitrile with 5 pmol d8-AEA (Cayman Chemical Company, Ann Arbor, Michigan, USA, #390050) in a borosilicate glass tube with a glass rod. Samples were sonicated, incubated overnight at −20 °C, and centrifuged at 1500 × g. Supernatants containing lipids were isolated, evaporated with nitrogen gas, washed with acetonitrile and evaporated with nitrogen gas again. Final reconstitution for liquid chromatography/tandem mass spectrometry was in 200 μl of acetonitrile.
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