Insulin Sensitivity Measurement by ITT

Insulin sensitivity was measured by performing an insulin tolerance test (ITT), and the rate constant for plasma glucose disappearance (Kitt) was calculated using a previously described method [5 (link), 27 (link)]. Recombinant human insulin (Novolin R, Novo Nordisk, Montes Claros, MG, Brazil) was administered at a dose of 2.0 U/kg, i.p. Blood samples were collected from the tail vein before (time 0) and 5, 10, 15, 20, 30 and 60 min after the insulin injection, and blood glucose levels were measured using standard test strips (One Touch UltraMini/Johnson & Johnson, Milpitas, CA, USA) [5 (link)]. The ITT is expressed as Kitt (%/min), i.e., the percent decrease in the plasma glucose concentration per minute [28 (link)].

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Speretta G.F., Ruchaya P.J., Delbin M.A., Melo M.R., Li H., Menani J.V., Sumners C., Colombari E., Bassi M, & Colombari D.S. (2019). Importance of AT1 and AT2 receptors in the nucleus of the solitary tract in cardiovascular responses induced by a high-fat diet. Hypertension Research, 42(4), 439-449.

Publication 2019

Novolin R One Touch UltraMini

Blood Blood glucose Glucose Human Insulin Insulin sensitivity Novolin Plasma Tail Tolerance Touch Vein

Corresponding Organization :

Other organizations : Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP), Southern Medical University, University of Florida

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Variable analysis

independent variables

Insulin dose (2.0 U/kg, i.p.)

dependent variables

Rate constant for plasma glucose disappearance (Kitt, %/min)

control variables

Recombinant human insulin (Novolin R, Novo Nordisk, Montes Claros, MG, Brazil)
Blood glucose measurement method (One Touch UltraMini/Johnson & Johnson, Milpitas, CA, USA)
Blood sampling timepoints (0, 5, 10, 15, 20, 30, 60 min)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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