Blood samples were taken at 0.25, 0.5, 1, 2, 4, 6, and 8 h after oral administration of auranofin or aurocyanide (Ott Joslin 2009 (link)). 20 μl of plasma samples were added to 180 μl of acetonitrile containing internal standard, vortexed for 5 min at 15,000 rpm. The supernatants were subjected to LC–MS/MS (Triple Quad 5500, Applied Biosystems, Foster City, CA, USA). Phoenix WinNonlin 6.4 version program (Pharsight Corporation, Mountain View, CA, USA) and non-compartmental analysis model were used for the calculation of pharmacokinetic parameters.
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Auranofin and Aurocyanide Pharmacokinetics
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Other organizations : Seoul National University, Hanyang University, Daegu-Gyeongbuk Medical Innovation Foundation, Chosun University
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Variable analysis
independent variables
- Oral administration of auranofin
- Oral administration of aurocyanide
- Blood sample concentrations at 0.25, 0.5, 1, 2, 4, 6, and 8 h
- 20 μl of plasma samples
- 180 μl of acetonitrile containing internal standard
- Vortexed for 5 min at 15,000 rpm
- LC–MS/MS (Triple Quad 5500, Applied Biosystems, Foster City, CA, USA)
- Phoenix WinNonlin 6.4 version program (Pharsight Corporation, Mountain View, CA, USA)
- Non-compartmental analysis model
- No positive or negative controls were explicitly mentioned in the provided information.
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