Compound Preparation for Cell Culture

We used 4OHT (H7904, Sigma) and doxycycline (D3447, Sigma) as described^{20 (link)}. Etoposide (E1383, Sigma) was dissolved in 50 mM DMSO, aliquoted and stored at −20 °C. Before use, Etoposide was added to fresh medium at a final concentration of 20 µM. Rapamycin (1292, Tocris) was dissolved in 100% ethanol at a 27.3 mM concentration, aliquoted and stored at −20 °C. Before use Rapamycin was added to fresh medium from an intermediate 27.3 μM solution at a final concentration of 20 nM. DMOG (D3695, Sigma) was dissolved in water at a 150 mM concentration, aliquoted and stored at −20 °C. Before use, DMOG was added to fresh medium at a final concentration of 1 mM. 1-Thioglycerol (M1753, Sigma) was stored at 4 °C. Before use, 1-Thioglycerol was added to fresh medium at a final concentration of 1 mM. Nutlin-3 (S1061, Selleckchem) was dissolved in DMSO at a 10 mM concentration, aliquoted and stored at −80 °C. Before use, Nutlin-3 was added to fresh medium at a final concentration of 10 μM.