Murine Aneurysm Induction Protocols

See Study approval for an ethics declaration. The PPE (MilliporeSigma) model was described various times before (13 (link), 44 (link)). Briefly, 10-week-old male C57BL/6J WT mice (Taconic Biosciences) on regular chow diet (R36; Lantmännen) were kept under standard conditions (temperature 22°C, humidity 56%). Similarly, AngII-minipump implantation for AAA induction has been described various times before (19 (link)). Briefly, 10-week-old ApoE^–/– male mice (Charles River Laboratories) received an AngII-loaded (Bachem) osmotic minipump (model 2004, DURECT Corporation) implanted subcutaneously. Anesthesia, PPE-AAA induction, AngII-AAA induction, murine B-mode ultrasound images, and protocols for euthanasia and tissue processing are discussed in the Supplemental Data.

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Busch A., Pauli J., Winski G., Bleichert S., Chernogubova E., Metschl S., Winter H., Trenner M., Wiegering A., Otto C., Fischer J., Reiser J., Werner J., Roy J., Brostjan C., Knappich C., Eckstein H.H., Paloschi V, & Maegdefessel L. (2021). Lenvatinib halts aortic aneurysm growth by restoring smooth muscle cell contractility. JCI Insight, 6(15), e140364.

Publication 2021

C57BL/6J WT mice ApoE–/– male mice

Anesthesia Apoe C57bl mice Euthanasia Humidity Implantation Male Mice Murine Osmotic Regular diet River Tissue Ultrasound

Corresponding Organization : German Centre for Cardiovascular Research

Other organizations : Karolinska Institutet, Vienna General Hospital, Medical University of Vienna, Universitätsklinikum Würzburg

Top 5 similar protocols

Variable analysis

independent variables

PPE (MilliporeSigma) model
AngII-minipump implantation for AAA induction

dependent variables

Murine B-mode ultrasound images

control variables

10-week-old male C57BL/6J WT mice (Taconic Biosciences) on regular chow diet (R36; Lantmännen)
Temperature 22°C, humidity 56%
10-week-old ApoE−/− male mice (Charles River Laboratories)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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