Cells were treated as specified and cell lysates were generated as previously described(12 (link)). AR (N-20, directed against amino acids 1–20), DNA-PK (Thermo Fisher Scientific, #MS-423-P), Vinculin (Sigma-Aldrich, #V9264–200UL), VAV3 (EMD Millipore, #07–464), Prex1 (EMD Millipore, #MABC178), Lamin B (Santa Cruz, #6217), pAKT (Cell Signaling, 9271S), AKT (Cell Signaling, 9272S), pS6 (Cell Signaling, 2211S), S6 (Cell Signaling, 2217S), PARP/cleaved PARP (Cell Signaling, 9542S) antibodies were used for immunoblotting.
Protocol detail
Cell Lysis and Immunoblot Analysis
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Corresponding Organization : Thomas Jefferson University
Other organizations : University of California, San Francisco, Kettering University, Memorial Sloan Kettering Cancer Center
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Variable analysis
independent variables
- Cell treatment
- Protein expression levels of AR (N-20, directed against amino acids 1–20), DNA-PK (Thermo Fisher Scientific, #MS-423-P), Vinculin (Sigma-Aldrich, #V9264–200UL), VAV3 (EMD Millipore, #07–464), Prex1 (EMD Millipore, #MABC178), Lamin B (Santa Cruz, #6217), pAKT (Cell Signaling, 9271S), AKT (Cell Signaling, 9272S), pS6 (Cell Signaling, 2211S), S6 (Cell Signaling, 2217S), PARP/cleaved PARP (Cell Signaling, 9542S)
- Cell lysates generation as previously described(12 (link))
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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