Cell Culture Protocols of Various Cell Lines

Human U251, 293T, and PANC1 cells, rhesus monkey FrhK4 cells, African Green Monkey Vero and CV1 cells, Dog D17 and DK cells, mouse Min6 cells, feline CRFK cells, bovine MDBK cells, and rat NRK cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) and antibiotics as described previously^{24 (link)}. Mouse iPSCs were generated from murine fibroblasts by lentiviral transduction of four reprogramming factors^{25 (link)} and cultured without feeder cells on Matrigel-coated plates (BD Bioscience) in ESGRO-2i medium (Millipore) supplemented with 45% Dulbecco’s modified Eagle’s medium (DMEM), 5% FCS, 25 U/ml penicillin, and 25 µg/ml streptomycin. Transgene-free iPSCs were reported previously^{26 (link)}, which were cultured in Pluriton medium (Stemgent, Cambridge, MA) supplemented with 25% mTeSR1 medium (Stemcell Technology) and 50 U/ml penicillin and 50 µg/ml streptomycin. Adipose-derived MSCs were derived and cultured as described previously^{27 (link)}.