Trigeminal Nerve Imaging Preparation

Trigeminal imaging preparation was performed as described previously (16 (link)). Epifluorescence imaging of the ganglia was performed using an upright microscope (FVMPE-RS, Olympus) equipped with a 4×, 0.28-numerical aperture air objective. Illumination was provided with a 130-W halogen light source (U-HGLGPS, Olympus), using a standard green excitation/emission filter cube. Images were acquired using an ORCA-Flash 4.0 CMOS camera (Hamamatsu, Japan) at a frame rate of 10 Hz using MetaMorph (Molecular Devices). pClamp software was used to trigger the imaging acquisition and to generate TTL pulses for synchronizing other instruments (videography and force recording) through a Digidata 1550 (Molecular Devices).

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Szczot M., Liljencrantz J., Ghitani N., Barik A., Lam R., Thompson J.H., Bharucha-Goebel D., Saade D., Necaise A., Donkervoort S., Foley A.R., Gordon T., Case L., Bushnell M.C., Bönnemann C.G, & Chesler A.T. (2018). PIEZO2 mediates injury-induced tactile pain in mice and humans. Science translational medicine, 10(462), eaat9892.

Publication 2018

FVMPE-RS U-HGLGPS ORCA-Flash 4.0 CMOS camera MetaMorph Digidata 1550

Cmos Devices Frame Ganglia Halogen Light Microscope Orca Pulses Trigger

Corresponding Organization : National Institutes of Health

Other organizations : National Institute of Neurological Disorders and Stroke, Children's National

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Variable analysis

independent variables

Trigeminal imaging preparation

dependent variables

Epifluorescence imaging of the ganglia

control variables

Upright microscope (FVMPE-RS, Olympus) equipped with a 4×, 0.28-numerical aperture air objective
130-W halogen light source (U-HGLGPS, Olympus), using a standard green excitation/emission filter cube
ORCA-Flash 4.0 CMOS camera (Hamamatsu, Japan) at a frame rate of 10 Hz using MetaMorph (Molecular Devices)
PClamp software used to trigger the imaging acquisition and to generate TTL pulses for synchronizing other instruments (videography and force recording) through a Digidata 1550 (Molecular Devices)

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