Mice were anesthetized with ketamine and xylazine. The spleen was identified after a midline laparotomy incision and removed after appropriate blood vessel ligation. Sham animals underwent laparotomy without splenectomy.
Protocol detail
Splenectomy Procedure in Mice
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Corresponding Organization :
Other organizations : Feinstein Institute for Medical Research, Northwell Health
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Variable analysis
independent variables
- Anesthesia with ketamine and xylazine
- Spleen removal (splenectomy)
- Sham animals underwent laparotomy without splenectomy
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