Mesenchymal stromal cells were generated from hiPSC (hiMSCs) of the OPG-XL hiPSCs (LUMC0103iOPG) and of the two thereof derived CRISPR/Cas9 isogenic control hiPSCs (B89 and C81) using Stemcell Technologies’ Mesenchymal Progenitor Kit following the manufacturer’s instructions. Chondrogenesis and osteogenesis was performed in organoids following our established protocol employing 750 000 cells per organoid as described before [25 (link)].
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