Biomarker Measurement in Fasting Samples

Information about personal and medical factors was obtained using standardized questionnaires. Prior to the intervention, fasting venous blood samples were collected in EDTA containers, immediately centrifuged and stored at -80°C. Serum creatinine, cystatin C, high-sensitivity C-reactive protein, and interleukin 6 (IL-6) were measured on a Beckman Coulter Unicel DxC600 Synchron Clinical System (Beckman Coulter, California). High-sensitivity troponin T (cTnT) and N-terminal proB-type natriuretic peptide (NT-proBNP) were assessed on the Elecsys 2010 immunoassay analyzer (F. Hoffmann–La Roche, Switzerland). The body surface was estimated by using Du Bois’s equation (Body surface area (m²) = [Weight (kg)^0.425 x height (m)^0.725] x 0.007184). The creatinine and cystatine C based chronic kidney disease epidemiology collaboration (CKD-EPI) formula was applied to estimate the glomerular filtration rate (eGFR).[17 (link)]