Two types of air dried material (inflorescence+leaves or stems) were separately subjected to hydrodistillation for 3 h using a Clevenger type apparatus to yield essential oils. Conventional hydrodistillation is considered the primary method for essential oil extraction.17 Although hydrodistillation could lead to artifacts at when performed at higher temperatures over long hydrodistillation times at low pH,18 (link) we only applied conditions accepted by the European Pharmacopoeia (European Directorate for the Quality of Medicines, Council of Europe, Strasbourg, France, 2014) and thus avoided these artifacts. Solutions of the essential oils in DMSO (10 mg/ml stock solutions) for biological evaluation and in n-hexane (10% w/v) for gas-chromatographic analysis.
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Essential Oil Extraction and Analysis
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Biological
Dmso
Essential oils
European
For gas chromatographic
Hexane
Higher temperatures
Inflorescence
Medicines
Stems
Corresponding Organization :
Other organizations : Montana State University, Institute of Plant Biology and Biotechnology, Anadolu University, Eskişehir City Hospital, Al-Farabi Kazakh National University, King Saud University
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Variable analysis
independent variables
- Type of air dried material (inflorescence+leaves or stems)
- Yield of essential oils
- Hydrodistillation time (3 h)
- Hydrodistillation method (Clevenger type apparatus)
- Conditions accepted by the European Pharmacopoeia
- Positive control: Not specified
- Negative control: Not specified
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