Schistosoma mansoni DNA and RNA Sequencing

S. mansoni clonal DNA was obtained from single miracidium infections of Biomphalaria snails. Male and female adults (NMRI strain, Puerto Rican origin) were obtained from infected C57Bl/6 mice. DNA extraction was performed and sequencing libraries were prepared as previously described [18] (link). Eight and lanes were sequenced for the male samples and one lane for the female sample, both as 108-base paired reads. For RNA-seq samples, total RNA samples were obtained from cercariae, 3 hours and 24 hours post-infection schistosomula, and 7-week old mixed sex adult worms. Schistosomula samples were obtained using mechanical transformation [19] (link). RNA-seq libraries were prepared using a modified version of the protocol described in [8] (link) and sequenced as 76-base paired reads. All samples were sequenced using the Illumina Genome Analyzer IIx platform. Raw sequence data were submitted to public data repositories; DNA reads were submitted to ENA http://www.ebi.ac.uk/ena/ under accession number ERP000385 and RNA-seq reads were submitted to ArrayExpress http://www.ebi.ac.uk/arrayexpress/ under accession number E-MTAB-451).