Plasma Clotting Time Measurement

Human platelet-poor plasma (PPP) was prepared and clotting time measured as previously described [4 (link)]. Plasma (50 μl) was incubated with HBS, or 5 μM SCP (50 μl) in HBS, for 3 min at 37°C in coagulometer cuvettes. Subsequently, 50 μl of CaCl₂ (8.3 mM final) and diluted Innovin (8 pM final TF concentration) were added, and time to clot formation was measured with a KC4 Coagulation Analyzer (Trinity Biotech, Ireland). In selected experiments, FXI- or FIX-depleted PPP was used.

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Puy C., Tucker E.I., Ivanov I.S., Gailani D., Smith S.A., Morrissey J.H., Gruber A, & McCarty O.J. (2016). Platelet-Derived Short-Chain Polyphosphates Enhance the Inactivation of Tissue Factor Pathway Inhibitor by Activated Coagulation Factor XI. PLoS ONE, 11(10), e0165172.

Publication 2016

KC4 Coagulation Analyzer

Clot Coagulation Human Innovin Plasma Platelet

Corresponding Organization : University of Illinois System

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Variable analysis

independent variables

Incubation of plasma (50 μl) with HBS
Incubation of plasma (50 μl) with 5 μM SCP in HBS

dependent variables

Time to clot formation

control variables

Incubation time (3 min)
Incubation temperature (37°C)
Concentration of CaCl2 (8.3 mM final)
Concentration of diluted Innovin (8 pM final TF concentration)

controls

Positive control: Not specified
Negative control: Incubation of plasma (50 μl) with HBS

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