Evaluating Cell Cycle and Apoptosis

Flow cytometric analysis (BD Accuri™ C6, BD biosciences) was performed to evaluate cell cycle distribution by propidium iodide (PI) staining, apoptosis by AnnexinV-FITC/PI staining, and to detect acidic vesicular organelles (AVOs) by acridine orange staining, as previously described
[39 (link), 40 (link)]. Active Caspase-3 Apoptosis Kit (BD biosciences) was used to detect the heterodimer of 17 and 12 kDa subunits, which is derived from the pro-enzyme.

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Del Bufalo D., Desideri M., De Luca T., Di Martile M., Gabellini C., Monica V., Busso S., Eramo A., De Maria R., Milella M, & Trisciuoglio D. (2014). Histone deacetylase inhibition synergistically enhances pemetrexed cytotoxicity through induction of apoptosis and autophagy in non-small cell lung cancer. Molecular Cancer, 13, 230.

Publication 2014

BD Accuri™ C6 Active Caspase-3 Apoptosis Kit

Acidic Acridine orange Apoptosis Caspase 3 Cell cycle Enzyme Fitc Flow cytometric Organelles Propidium iodide Subunits

Corresponding Organization :

Other organizations : Istituto Superiore di Sanità, National Cancer Institute

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Experimental treatments or conditions that were manipulated by the researchers to evaluate their effects on the dependent variables

dependent variables

Cell cycle distribution
Apoptosis
Acidic vesicular organelles (AVOs)
Active Caspase-3

control variables

Experimental procedures and methods, such as the use of the BD Accuri™ C6 flow cytometer and the specific staining protocols (propidium iodide, AnnexinV-FITC/PI, acridine orange) as previously described in the referenced studies [39, 40]

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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