One hind limb of a rat was subjected to mechanical vibration with a laboratory vortex mixer, as previously described.2 (link),7 (link),9 (link),10 (link) Rats were anesthetized with 2.5% isoflurane in oxygen and their right hind limb affixed to the platform of a vortex mixer (Digital Vortex Genie II; Thermo Fisher Scientific, Waltham, MA) with Micropore surgical tape (3M Health Care, St. Paul, MN) so that the knee and ankle joint were both at 90°, without rotational torque on the leg. Each hind limb was vibrated at a frequency of 60 to 80 Hz with a 5 mm peak-to-peak displacement amplitude for 15 minutes. These vibration frequencies are within the range produced by hand-held power tools (35–150 Hz).36 (link)
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Mechanical Vibration of Rat Hind Limb
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Corresponding Organization : University of California, San Francisco
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Variable analysis
independent variables
- Mechanical vibration with a laboratory vortex mixer on one hind limb of a rat
- Outcomes measured from the vibrated hind limb
- Anesthesia with 2.5% isoflurane in oxygen
- Affixing the right hind limb to the platform of the vortex mixer with Micropore surgical tape so that the knee and ankle joint were both at 90°, without rotational torque on the leg
- Vibration frequency of 60 to 80 Hz with a 5 mm peak-to-peak displacement amplitude for 15 minutes
- Positive control: None mentioned
- Negative control: None mentioned
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