Western blot was carried out as previously described [13 (link)]. The following primary antibodies were used (See also Supplementary Table): anti LOXL2 [20 (link)] 1:10,000, ER, GAPDH, Lamin, and β-Tubulin (1:500; Santa-Cruz). Anti-E-Cadherin (1:500), Vimentin (1:2000), HIF-1α (1:2000) and Fibronectin (1:1000) (abcam). Horseredish peroxidase-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch Laboratories).
Free full text: Click here