Zinc oxide nanoparticles were
synthesized according to a previously described protocol with slight
modifications. Briefly, 6.0 g of zinc acetate dihydrate (Zn(NO3)2·2H2O) (Sigma-Aldrich) was added
to 100 mL of extract and kept on a magnetic stirrer at 60 °C
for 2 h. Once the reaction was complete, the mixture was allowed to
cool down at 25 °C and centrifuged (HERMLE Z326K) at 10 000
rpm for 10 min. The supernatant was discarded, and the remaining pellet
was washed thrice with distilled water, poured into a clean Petri
plate, and oven-dried at 90 °C. The dried material was then ground
into fine powder in a pestle and mortar and calcined for 2 h at 500
°C to remove any impurities. The annealed powder was stored in
an airtight glass vial, labeled as ZnO-NPs, and was further used for
physical characterizations and biological applications.28 (link)
synthesized according to a previously described protocol with slight
modifications. Briefly, 6.0 g of zinc acetate dihydrate (Zn(NO3)2·2H2O) (Sigma-Aldrich) was added
to 100 mL of extract and kept on a magnetic stirrer at 60 °C
for 2 h. Once the reaction was complete, the mixture was allowed to
cool down at 25 °C and centrifuged (HERMLE Z326K) at 10 000
rpm for 10 min. The supernatant was discarded, and the remaining pellet
was washed thrice with distilled water, poured into a clean Petri
plate, and oven-dried at 90 °C. The dried material was then ground
into fine powder in a pestle and mortar and calcined for 2 h at 500
°C to remove any impurities. The annealed powder was stored in
an airtight glass vial, labeled as ZnO-NPs, and was further used for
physical characterizations and biological applications.28 (link)
