Immunoblotting of RBM4, α-tubulin, and GAPDH

Immunoblotting was performed as described (Lin and Tarn, 2011 (link)). We used affinity-purified polyclonal antibodies against RBM4 (Lin and Tarn, 2011 (link)), α-tubulin (GenScript, Piscataway, NJ), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Proteintech, Rosemont, IL), and FLAG (Sigma-Aldrich).

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Tarn W.Y., Kuo H.C., Yu H.I., Liu S.W., Tseng C.T., Dhananjaya D., Hung K.Y., Tu C.C., Chang S.H., Huang G.J, & Chiu I.M. (2016). RBM4 promotes neuronal differentiation and neurite outgrowth by modulating Numb isoform expression. Molecular Biology of the Cell, 27(10), 1676-1683.

Publication 2016

α-tubulin GAPDH FLAG

Antibodies affinity Gapdh Glyceraldehyde 3 phosphate dehydrogenase α tubulin

Corresponding Organization :

Other organizations : Institute of Biomedical Sciences, Academia Sinica, Chang Gung University, National Health Research Institutes

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Variable analysis

independent variables

Antibodies used for immunoblotting: affinity-purified polyclonal antibodies against RBM4, α-tubulin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and FLAG

dependent variables

Protein expression levels of RBM4, α-tubulin, GAPDH, and FLAG detected by immunoblotting

control variables

Immunoblotting protocol as described in the referenced publication (Lin and Tarn, 2011)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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