The semi-synthetic drug amitozyn was prepared as described previously at a concentration of 25 mg/mL [15 (link)]. CQ, pyrimethamine, ART, monoclonal mouse anti-α-tubuline (T9026), and polyclonal anti-γ-tubuline antibodies (T3559) were purchased from Sigma. Advanced RPMI Medium 1640, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-rabbit antibodies were from Invitrogen. 4′,6-Diamidino-2-phenylindole, dihydrochloride (DAPI) and LDH cytotoxicity kit were from Termo Scientific Pierce. EGM-2 medium was from Lonza. The polyclonal rabbit anti-human RBC antibodies were from Rockland. Human blood O+ and AB human serum were purchased from Valley Biomedical.
Human HeLa, KB3, HT29, HCT116, A549, IMR90, HUVEC, MESSA, and murine B16, GL26 and COS7 cell lines were purchased from ATCC. HCT116 p53(−/−) cells with homozygous knock-out of p53 were kindly provided by Dr D Skoufias (IBS, Grenoble, France). Taxol-resistant A549T12 cells were obtained with permission from Dr S Horwitz (Albert Einstein College of Medicine, New York, NY, USA). The MESSA Dx5 cells were kindly provided by Dr L Lafanechère (CNRS, UMR 5168/CEA/IRTSV, Grenoble, France).
Human HeLa, KB3, HT29, HCT116, A549, IMR90, HUVEC, MESSA, and murine B16, GL26 and COS7 cell lines were purchased from ATCC. HCT116 p53(−/−) cells with homozygous knock-out of p53 were kindly provided by Dr D Skoufias (IBS, Grenoble, France). Taxol-resistant A549T12 cells were obtained with permission from Dr S Horwitz (Albert Einstein College of Medicine, New York, NY, USA). The MESSA Dx5 cells were kindly provided by Dr L Lafanechère (CNRS, UMR 5168/CEA/IRTSV, Grenoble, France).
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