For immunoblotting of SF3B1/FLAG-SF3B1proteins in transiently-transfected HEK293T and stably-transduced TF1 and K562 cells in
which transcriptome analysis was done in parallel, Western blotting using a mouse
anti-human-SF3B1 antibody (Abcam #172634) at 1:1000 dilution was used.
Immunoblotting following affi nity purification of SF3B1 in HEK293T and TF1 cells was
performed as previously described, and primary antibodies were: anti-SF3B1 (Bethyl
Laboratories, A300–996A, 1:1,000), anti-ACTIN (Sigma, A2066, 1:2,000),
anti-DYKDDDDK (GenScript, A00187, 1:1,000), anti-SUGP1 (Bethyl
Laboratories A304–675A-M, 1:1,000), and anti-PHF5A (Proteintech 15554–1-AP,
1:1000)17 (link). Secondary antibodies
were: Donkey anti-Rabbit IgG (LI-COR, 926–68073, 1:5,000) and Goat anti-Mouse IgG
(LI-COR, 926–32210, 1:5,000).
which transcriptome analysis was done in parallel, Western blotting using a mouse
anti-human-SF3B1 antibody (Abcam #172634) at 1:1000 dilution was used.
Immunoblotting following affi nity purification of SF3B1 in HEK293T and TF1 cells was
performed as previously described, and primary antibodies were: anti-SF3B1 (Bethyl
Laboratories, A300–996A, 1:1,000), anti-ACTIN (Sigma, A2066, 1:2,000),
anti-DYKDDDDK (GenScript, A00187, 1:1,000), anti-SUGP1 (Bethyl
Laboratories A304–675A-M, 1:1,000), and anti-PHF5A (Proteintech 15554–1-AP,
1:1000)17 (link). Secondary antibodies
were: Donkey anti-Rabbit IgG (LI-COR, 926–68073, 1:5,000) and Goat anti-Mouse IgG
(LI-COR, 926–32210, 1:5,000).
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