Cytokine Profiling in Viral Pneumonia

Relative expression of proinflammatory and immunomodulatory mediators in BAL fluid of mice inoculated with a lethal vs. sublethal dose of PVM (day 7) and mice inoculated with a sublethal dose of PVM alone (day 7 vs. day 21) was evaluated using the Proteome Profiler Array, Panel A and the Mouse Angiogenesis Profiler Array (both from R&D Systems, Minneapolis, MN, USA) as previously described [33 (link)]. Each profiler membrane was probed with 500 µL BAL fluid (100 µL from each of 5 mice per group). Raw data were normalized to levels of each cytokine detected in BAL fluid samples from uninfected controls. Quantitative evaluation of proinflammatory cytokines in BAL fluid was carried out by ELISA (DuoSet, R&D Systems).

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Limkar A.R., Percopo C.M., Redes J.L., Druey K.M, & Rosenberg H.F. (2021). Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice. Viruses, 13(5), 728.

Publication 2021

Proteome Profiler Array Panel A

Angiogenesis Cytokines Elisa Immunomodulatory Membrane Mice Proteome

Corresponding Organization : National Institute of Allergy and Infectious Diseases

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Variable analysis

independent variables

Dose of PVM (lethal vs. sublethal)

dependent variables

Relative expression of proinflammatory and immunomodulatory mediators in BAL fluid
Quantitative evaluation of proinflammatory cytokines in BAL fluid

control variables

BAL fluid samples from uninfected controls

controls

Positive control: None specified
Negative control: BAL fluid samples from uninfected mice

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