Purified KTs (100 μg/ml) from both WaF17.12 and WaATCC 96603 were pre-incubated with 25 µM of castanospermine (Sigma-Aldrich), a β-glucanase inhibitor, for 1 h at 25 °C under static conditions [26 (link)] and, then, added to the P. berghei cultures, in triplicates. The inhibitory effect of the indolizine alkaloid on the antimicrobial activity of KTs was evaluated on P. berghei sporogonic stage cultures after 24 h incubation at 19 °C and it was indicated as the average of three replicates. Upon castanospermine treatment, the number of parasites was compared to controls.
Free full text: Click here
