Acquisition and Synthesis of Small Molecule Inhibitors
Novartis 224 (HY-18717), Novartis 556 (HY-13972), Agios 135 (HY-12475), and AG-221 (HY-18690) were purchased from Medchem Express. AGI-5198 (14624) and AGI-6780 (14639) were purchased from Cayman Chemicals. IDH2-C100 was purchased from XcessBio. AG-120 was purchased from CG Biopharma. GSK864 was a gift from Structure Genomics Consortium24 (link). ML309 was synthesized as previously described20 (link). The synthesis of Sanofi 1 and Novartis 530 are reported in the supplemental methods.
Urban D.J., Martinez N.J., Davis M.I., Brimacombe K.R., Cheff D.M., Lee T.D., Henderson M.J., Titus S.A., Pragani R., Rohde J.M., Liu L., Fang Y., Karavadhi S., Shah P., Lee O.W., Wang A., McIver A., Zheng H., Wang X., Xu X., Jadhav A., Simeonov A., Shen M., Boxer M.B, & Hall M.D. (2017). Assessing inhibitors of mutant isocitrate dehydrogenase using a suite of pre-clinical discovery assays. Scientific Reports, 7, 12758.
Kept constant to ensure valid results (not explicitly mentioned)
positive controls
GSK864 (a gift from Structure Genomics Consortium)
negative controls
Not explicitly mentioned
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