Individual sera were titrated in parallel for the presence of VSV‐specific IgG by end‐point ELISA. Neutralizing dose 50 were measured as described (Sammicheli et al, 2016 (link)).
SARS-CoV-2 Antibody Titration by ELISA
Individual sera were titrated in parallel for the presence of VSV‐specific IgG by end‐point ELISA. Neutralizing dose 50 were measured as described (Sammicheli et al, 2016 (link)).
Corresponding Organization :
Other organizations : Vita-Salute San Raffaele University, Istituti di Ricovero e Cura a Carattere Scientifico, Istituto Nazionale Genetica Molecolare, University of Naples Federico II, Istituto Nazionale di Fisica Nucleare, Sezione di Cagliari, University of Milan, Sapienza University of Rome, University of Iowa
Variable analysis
- Dilution of sera (1/20, 1/500, 1:10 up to 1/1,280 or 1/32,000)
- Presence of SARS-CoV-2 S1 RBD-specific antibody measured by end-point ELISA
- Presence of VSV-specific IgG measured by end-point ELISA
- Neutralizing dose 50
- Concentration of recombinant SARS-CoV-2 S1 subunit protein used to coat ELISA plates (2 μg/ml)
- Blocking buffer composition (3% fat-free milk and 0.05% Tween20 in PBS)
- Incubation times and temperatures
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
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