Mongoose Gut Microbiome Analysis

The culturomics data acquired in this study was compared with the data obtained from the same specimens by a microbial profiling approach [10 (link)]. Microbial profiling was performed as described elsewhere [10 (link)]. Briefly, the DNA was extracted from 500 mg of feces of each mongoose using the NZYSoil gDNA isolation kit (NZYTech, Lisbon, Portugal), followed by the amplification of the full-length 16S rRNA gene, and subsequently, the amplicons were sequenced on the PacBio SMRT RS-II platform (Pacific Biosciences, Menlo Park, CA, USA; commercially available at Eurofins Genomics, München, Germany). Data were preprocessed using the PacBio SMRT Analysis Portal (Pacific Biosciences, Menlo Park, CA, USA), generating single-molecule circular consensus sequences (CCS) that were analyzed using the EzBioCloud platform (Seoul, Korea).