Rabbit polyclonal (L75423) and mouse monoclonal (LC3B-6) antibodies against LC3B were purchased from Sigma-Aldrich. Rabbit polyclonal antibodies against p62 (H-240) and Beclin 1 (H-300), as well as, a mouse monoclonal antibody against actin (C-4) were purchased from Santa Cruz Biotechnology. An additional rabbit polyclonal antibody against p62 was purchased from Cell Signaling (#5114). Rabbit polyclonal antibodies specific for N, M and RdRp BEV proteins, as well as a rat polyclonal antibody specific for Mpro were previously described [15 (link), 19 (link), 56 (link)]. Horseradish peroxidase-conjugated and Alexa Fluor-conjugated secondary antibodies were purchased from Sigma-Aldrich and Invitrogen, respectively.
3-Methyladenine (10 mM; 3-MA), wortmannin (5 μM; Wn), Earle´s balanced salt solution (EBSS), ammonium chloride (20 mM; NH4CL), thapsigargin (200 nM; TG) and dithiothreitol (2 mM; DTT) were purchased from Sigma-Aldrich. Hydroxychloroquine (17 μM; HCQ) was purchased from Sanofi-Synthelabo. Epoxomicin (200 nM; Epox) was purchased from Peptanova.
3-Methyladenine (10 mM; 3-MA), wortmannin (5 μM; Wn), Earle´s balanced salt solution (EBSS), ammonium chloride (20 mM; NH4CL), thapsigargin (200 nM; TG) and dithiothreitol (2 mM; DTT) were purchased from Sigma-Aldrich. Hydroxychloroquine (17 μM; HCQ) was purchased from Sanofi-Synthelabo. Epoxomicin (200 nM; Epox) was purchased from Peptanova.
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