Total RNA was isolated from the cell pellet resuspended in TRIzol reagent (Invitrogen, CA) as described previously (Yang et al., 2009b (link)). Briefly, RNase-free DNase I (Ambion, Austin, TX, United States) was used for each total RNA sample to digest residual genomic DNA, total RNA was subsequently purified using the RNeasy Mini Kit (Qiagen, CA). NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, United States) was used to quantify the total cellular RNA, and Agilent Bioanalyzer (Agilent, CA) was used to assess RNA quality. Purified RNA with high quality was then used as the template to generate ds-cDNA using Invitrogen ds-cDNA synthesis kit (Invitrogen, CA).
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