Measurement of Intracellular and Mitochondrial ROS

Intracellular reactive oxygen species (iROS) were determined using the fluorescent dye 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Molecular Probes, Eugene, OR, USA), and mitochondrial-specific ROS were measured by MitoSOX Red (Molecular Probes). Briefly, dual-biofilm cells treated with and without 12.5 μg mL⁻¹ of MYR after centrifugation at 13,000× g for 5 min, were treated with 10 mM H₂DCFDA for 1 h, or 5 M MitoSOX Red (Molecular Probes), for 30 min at 37 °C. The fluorescent cells were measured with the FACS Verse microplate reader [15 (link)].

Free full text: Click here

Maione A., La Pietra A., de Alteriis E., Mileo A., De Falco M., Guida M, & Galdiero E. (2022). Effect of Myrtenol and Its Synergistic Interactions with Antimicrobial Drugs in the Inhibition of Single and Mixed Biofilms of Candida auris and Klebsiella pneumoniae. Microorganisms, 10(9), 1773.

Publication 2022

2′,7′-dichlorofluorescein diacetate (DCFH-DA) MitoSOX Red

Biofilm Cells Centrifugation Dye 2 Fluorescent dye H2dcfda Intracellular Mitochondrial Molecular probes Reactive oxygen species

Corresponding Organization : University of Naples Federico II

Other organizations : Istituto Nazionale Biostrutture e Biosistemi

Top 5 similar protocols

Variable analysis

independent variables

Treatment with 12.5 μg mL^-1 of MYR

dependent variables

Intracellular reactive oxygen species (iROS) measured using the fluorescent dye 2',7'-dichlorofluorescein diacetate (DCFH-DA)
Mitochondrial-specific ROS measured using MitoSOX Red

control variables

Dual-biofilm cells
Centrifugation at 13,000× g for 5 min
Treatment with 10 mM H2DCFDA for 1 h
Treatment with 5 μM MitoSOX Red for 30 min at 37 °C
Measurement with the FACS Verse microplate reader

controls

Dual-biofilm cells treated without 12.5 μg mL^-1 of MYR

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!