HCT-116, SW620, HT29, SW480, DLD-1 cells were obtained from American Type Culture Collection (ATCC, Manassas VA, USA), and MC-38 cells from Dr. Shoshana Yakar. Cells were maintained in DMEM or RPMI with 10%FBS and 1%penicillin/streptomycin at 37°C and 5%CO2. CAF were isolated as described13 (link). BMDM were derived from bone marrow and induced to differentiate with M-CSF for 7 days. Organoid cultures of small intestinal crypts were established as described13 (link).
NT157 was synthesized by Dr. Salim Joubran from the Jerusalem laboratory and used as described35 (link). LPS and IGF-1 were purchased from Sigma-Aldrich (St. Louis, MO, USA); and IL-6 from Goldbio.(St. Louis, MO, USA)
NT157 was synthesized by Dr. Salim Joubran from the Jerusalem laboratory and used as described35 (link). LPS and IGF-1 were purchased from Sigma-Aldrich (St. Louis, MO, USA); and IL-6 from Goldbio.(St. Louis, MO, USA)
