Fabrication of Chronic Dopamine Microsensors

Dopamine microsensors for chronic implantation consisted of carbon-fiber microelectrodes insulated in a fused-silica capillary20 (link),21 (link). A single carbon fiber (34–700, Goodfellow Corporation, PA) was inserted into a 10–15 mm length of fused silica (Polymicro Technologies, AZ) while submerged in 2-propanol. One end of the microsensor was then sealed with Devcon two-component epoxy (IWT Performance Polymers, FL) and allowed to dry, leaving a length of carbon fiber protruding. A silver connector (Newark, IL) was secured in contact with the carbon fiber on the other end of the silica with silver epoxy (8331; MG Chemicals, BC, Canada), allowed to cure overnight, then insulated with a layer of two component epoxy. After an additional twelve hours of drying, the fabrication of the chronic microsensor was finalized by trimming the exposed carbon fiber to the desired length of the sensor (150–200 µm).

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Clark J.J., Sandberg S.G., Wanat M.J., Gan J.O., Horne E.A., Hart A.S., Akers C.A., Parker J.G., Willuhn I., Martinez V., Evans S.B., Stella N, & Phillips P.E. (2009). Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals. Nature methods, 7(2), 126-129.

Publication 2009

Carbon fiber Dopamine Epoxy Implantation Microelectrodes Polymers Propanol Silica Silver

Corresponding Organization :

Other organizations : University of Washington

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Variable analysis

independent variables

Length of carbon fiber protruding (150-200 μm)

dependent variables

Chronic implantation of dopamine microsensors

control variables

Carbon-fiber microelectrode insulated in a fused-silica capillary
Single carbon fiber (34–700, Goodfellow Corporation, PA) inserted into a 10–15 mm length of fused silica (Polymicro Technologies, AZ) while submerged in 2-propanol
One end of the microsensor sealed with Devcon two-component epoxy (IWT Performance Polymers, FL) and allowed to dry
Silver connector (Newark, IL) secured in contact with the carbon fiber on the other end of the silica with silver epoxy (8331; MG Chemicals, BC, Canada), allowed to cure overnight, then insulated with a layer of two component epoxy

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