FA contents were measured as previously described (26 (link)) with some modifications. About 500 to 1000 age-synchronized adult worms were washed off plates and washed three times with water. Worm pellets were resuspended with 1.2 ml of 2.5% sulfuric acid in methanol and incubated at 80 °C for 1 h. Then, 1 ml of supernatant was mixed with 1.2 ml of hexane and 1.8 ml of water to extract FA methyl esters (FAMEs) for GC-MS analysis. The Supelco 37 Component FAME Mix (Sigma) was used to determine the retention time. The Shimadzu GCMS-TQ8040 Gas Chromatograph Mass Spectrometer equipped with SH-Rxi-5sil MS column was used.
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