HCT116, HT29, HCT116/R, and HT29/R (5X103 cells/well) were seeded in 96-well plates, and treated with 1, 2, 4, 8, 16, 32, 64, or 128 μM oxaliplatin for 24 hours. Cells were then incubated with CCK8 (Cell Counting Kit-8) (Dojindo, Tokyo, Japan) for another 2 hours, and the absorbance at 450 nm was measured using Microplate Autoreader (Thermo Fisher) according to previous study [15 (link)]. Moreover, HCT116/R and HT29/R cells were seeded in 96-well plates, and then transfected with shNC, shWDR62-1# or shWDR62-2# (GenePharma, Suzhou, China) using Lipofectamine 2000 (Gibco, Carlsbad, CA, USA) for 48 hours. Cells were then also subjected to CCK8 assay to detect the absorbance at 450 nm.
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Oxaliplatin Cytotoxicity in CRC Lines
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Corresponding Organization : Ningbo University
Other organizations : Ningbo No.6 Hospital
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Variable analysis
independent variables
- Oxaliplatin concentrations (1, 2, 4, 8, 16, 32, 64, or 128 μM)
- Transfection conditions (shNC, shWDR62-1#, shWDR62-2#)
- Cell viability/proliferation as measured by absorbance at 450 nm using the CCK8 assay
- Cell lines (HCT116, HT29, HCT116/R, HT29/R)
- Cell seeding density (5 x 10^3 cells/well)
- Incubation time (24 hours for oxaliplatin treatment, 48 hours for transfection)
- Positive control: Not specified
- Negative control: shNC (non-targeting control shRNA)
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