The control group (CON, n=8) was fed a standard ad libitum diet, consisting of dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]).
For the high-fat diet groups, food was presented from 9:00am to 10:00am each day. Animals were fed a daily diet of one can of Hill’s Prescription Diet (415g; 10% carbohydrate, 9% protein 8% fat, 0.3% fiber and 73% moisture [Hill’s Pet Nutrition, Topeka KS, USA]) and 825g dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]), supplemented with either 6g/kg of rendered pork fat (LARD, n=8) or salmon oil (SO, n=8). These alternative diets contained identical macronutrient content consisting of 21,025kJ/day comprised of 27% carbohydrate, 19% protein and 53% fat. The added lard consisted of 38.5% saturated fatty acid, 10.8% polyunsaturated fatty acid (10% ω-6; 0.8% ω-3), 44.6% monounsaturated fatty acid, and 0.1% cholesterol. The Salmon oil addition consists of 19.9% saturated fatty acid, 40.4% polyunsaturated fatty acid (1.5% ω-6; 35.5% ω-3).
The acute effects of six weeks of LARD and SO diets on insulin sensitivity and cardiac function have been reported previously (15 ). The present study reports data on extended diet durations of 3.0±0.3 months of lard feeding and 3.9±0.1 months of salmon oil feeding.
For the high-fat diet groups, food was presented from 9:00am to 10:00am each day. Animals were fed a daily diet of one can of Hill’s Prescription Diet (415g; 10% carbohydrate, 9% protein 8% fat, 0.3% fiber and 73% moisture [Hill’s Pet Nutrition, Topeka KS, USA]) and 825g dry chow (40% carbohydrate, 26% protein, 14% fat and 3% fiber [mixture of Laboratory HDL Canine Diet and Prolab Canine 2000, Richmond IN, USA]), supplemented with either 6g/kg of rendered pork fat (LARD, n=8) or salmon oil (SO, n=8). These alternative diets contained identical macronutrient content consisting of 21,025kJ/day comprised of 27% carbohydrate, 19% protein and 53% fat. The added lard consisted of 38.5% saturated fatty acid, 10.8% polyunsaturated fatty acid (10% ω-6; 0.8% ω-3), 44.6% monounsaturated fatty acid, and 0.1% cholesterol. The Salmon oil addition consists of 19.9% saturated fatty acid, 40.4% polyunsaturated fatty acid (1.5% ω-6; 35.5% ω-3).
The acute effects of six weeks of LARD and SO diets on insulin sensitivity and cardiac function have been reported previously (15 ). The present study reports data on extended diet durations of 3.0±0.3 months of lard feeding and 3.9±0.1 months of salmon oil feeding.
