Immunohistochemistry for CSF1, Cre, and eGFP

Immunohistochemistry, imaging, and quantification were performed as previously described [40 (link)]. Primary antibodies included rabbit anti-CSF1 (ab233387; Abcam), rabbit anti-Cre (69050; Novagen), or chicken anti-eGFP (ab13970; Abcam). Secondary antibodies included Alexa Fluor 488 donkey anti-rabbit (Jackson ImmunoResearch, 711-545-152), Alexa Fluor 555 donkey anti-rabbit (A32794, Invitrogen), or Alexa Fluor 488 donkey anti-chicken (703-545-155, Jackson ImmunoResearch).

Free full text: Click here

Patel R.R., Wolfe S.A., Borgonetti V., Gandhi P.J., Rodriguez L., Snyder A.E., D’Ambrosio S., Bajo M., Domissy A., Head S., Contet C., Dayne Mayfield R., Roberts A.J, & Roberto M. (2022). Ethanol withdrawal-induced adaptations in prefrontal corticotropin releasing factor receptor 1-expressing neurons regulate anxiety and conditioned rewarding effects of ethanol. Molecular Psychiatry, 27(8), 3441-3451.

Publication 2022

ab233387 ab13970 Alexa Fluor 488 donkey anti-rabbit Alexa Fluor 555 donkey anti-rabbit A32794 Alexa Fluor 488 donkey anti-chicken

Alexa fluor 488 Alexa fluor 555 Antibodies Chicken Csf1 Donkey Immunohistochemistry Rabbit

Corresponding Organization :

Other organizations : Scripps Research Institute, The University of Texas at Austin

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies: rabbit anti-CSF1 (ab233387; Abcam), rabbit anti-Cre (69050; Novagen), or chicken anti-eGFP (ab13970; Abcam)

dependent variables

Immunohistochemistry, imaging, and quantification

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!