Animals were anesthetized i.m. with 100 mg/kg ketamine and 10 mg/kg xylazine, and subjected to sham surgery (sham) or left cervical vagotomy with electrical stimulation of the distal nerve trunk. In sham-operated animals, the vagal trunk was exposed but not divided. The nerve trunk was placed across a bipolar platinum electrode (Plastics One) connected to a stimulation module (STM100A) controlled by AcqKnowledge software (Biopac Systems). Stimulation (1 V, 2 ms, 5 Hz) was applied for 10 min before and after endotoxin administration.
Protocol detail
Electrical Vagal Stimulation in Endotoxemia
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Corresponding Organization :
Other organizations : Feinstein Institute for Medical Research, Northwell Health
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Variable analysis
independent variables
- Sham surgery (sham) or left cervical vagotomy with electrical stimulation of the distal nerve trunk
- Not explicitly mentioned
- Anesthesia with 100 mg/kg ketamine and 10 mg/kg xylazine
- Exposure of the vagal trunk but not division (in sham-operated animals)
- Placement of the nerve trunk across a bipolar platinum electrode connected to a stimulation module
- Stimulation (1 V, 2 ms, 5 Hz) applied for 10 min before and after endotoxin administration
- Sham-operated animals (negative control)
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