Cryo-SEM Imaging of CaCO3 Emulsions

Cryo-scanning electron microscopy (Helios NanoLab G3 UC, FEI, USA) was employed on the cross-sectional and interfacial structure of CaCO₃ S/O/W emulsions. Samples were placed in liquid nitrogen and then transferred to a cryo-preparation chamber (PP3010T, Quorum Technologies, UK) under vacuum. After freeze-fracturing and high vacuum sublimation to sublimation at −95°C for 10 min to unbound water, and then the samples were sputter-coated with platinum and imaged using SEM. The observation was carried out at a distance between 3 and 5 mm with TLD detection at 2 kV (22 (link)).

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Zhang J., Cao Y, & Xu D. (2022). Encapsulation of calcium carbonate with a ternary mixture of sodium caseinate/gelatin/xanthan gum to enhance the dispersion stability of solid/oil/water emulsions. Frontiers in Nutrition, 9, 1090827.

Publication 2022

NanoLab G3 UC PP3010T

Caco3 Emulsions Nitrogen Platinum Scanning electron microscopy Vacuum

Corresponding Organization : Beijing Technology and Business University

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Variable analysis

independent variables

Type of sample (cross-sectional and interfacial structure of CaCO3 S/O/W emulsions)

dependent variables

Observed microstructure and morphology of the samples

control variables

Cryo-scanning electron microscopy (Helios NanoLab G3 UC, FEI, USA) parameters:
- Distance between sample and detector: 3-5 mm
- Accelerating voltage: 2 kV
- Detector: TLD
Sample preparation steps:
- Samples placed in liquid nitrogen
- Transferred to cryo-preparation chamber (PP3010T, Quorum Technologies, UK) under vacuum
- Freeze-fracturing and high vacuum sublimation at -95°C for 10 min to remove unbound water
- Samples sputter-coated with platinum

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