Flow cytometry was performed following the method reported by Xu et al. [21 (link)]. A single-cell suspension of the renal cells was prepared and incubated with a primary antibody or the appropriate isotype control antibody at 4 °C for 30 min. The following antibodies were used: FITC-conjugated anti-mouse CD45 (Biolegend), APC-conjugated anti-mouse Collagen I (Biolegend), FITC-conjugated anti-mouse CD4 (eBioscience), and PE-cy7-conjugated anti-mouse IL-17A (BioGems) antibodies. All flow cytometric analyses were performed using an LSR II Flow Cytometer (Beckman–Coulter) and the Flowjo software.
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Renal Cell Flow Cytometry Analysis
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Corresponding Organization : Tongji Hospital
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Variable analysis
independent variables
- Primary antibody
- Isotype control antibody
- Fluorescence intensity of FITC-conjugated anti-mouse CD45
- Fluorescence intensity of APC-conjugated anti-mouse Collagen I
- Fluorescence intensity of FITC-conjugated anti-mouse CD4
- Fluorescence intensity of PE-cy7-conjugated anti-mouse IL-17A
- Single-cell suspension of renal cells
- Incubation at 4 °C for 30 min
- Flow cytometric analysis using LSR II Flow Cytometer and Flowjo software
- Isotype control antibody
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