APOE Genotyping from Peripheral Blood

Genomic DNA was extracted from peripheral blood by using a Blood Genomic DNA Extraction Kit (Qiagen NV, Venlo, the Netherlands). Multiplex amplification refractory mutation system polymerase chain reaction was used to determine the APOE genotype. According to the method previously described,23 (link) these subjects were divided into three groups, E2 (e2/e2 and e2/e3), E3 (e3/e3), and E4 (e2/e4, e3/e4, and e4/e4).24 (link)

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Li W., Qiu Q., Sun L., Yue L., Wang T., Li X, & Xiao S. (2017). Sex differences in obesity and cognitive function in a cognitively normal aging Chinese Han population. Neuropsychiatric Disease and Treatment, 13, 2405-2410.

Publication 2017

Blood Genomic DNA Extraction Kit

Apoe Blood Genomic Genotype Multiplex polymerase chain reaction Mutation

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Shanghai Mental Health Center

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Variable analysis

independent variables

APOE genotype

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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